video fear conditioning apparatus  (Med Associates Inc)

Journal: Cell reports

Article Title: Entorhinal cortical delta oscillations drive memory consolidation

doi: 10.1016/j.celrep.2023.113267

Figure Lengend Snippet: (A) Schematic showing microinfusion before sleep recordings. Mice were infused either with the vehicle (saline) or the HCN channel antagonist ZD7288 (6.6 mM) just before recordings during the light phase. (B) Example time course of behavioral state (S, sleep; W, waking) and spectrogram from recordings after saline or ZD7288 injection. (C) The average delta power after saline or ZD7288 injection (paired samples t test, p = 0.033, n = 3). (D) Schematic showing microinfusion after the fear conditioning acquisition but before consolidation during sleep. (E) Percentage of freezing during baseline (BL), intertrial interval 1 (ITI1), ITI2, and ITI3 of saline- and ZD7288-infused mice on the training day. The black and blue lines indicate the average percentage of freezing of saline-infused mice (n = 9) and ZD7288-infused mice (n = 11), respectively (two-way mixed ANOVA followed by post hoc tests with Holm corrections; BL, saline vs. ZD, p = 1.000; ITI1, saline vs. ZD, p = 1.000; ITI2, saline vs. ZD, p = 0.339; ITI3, saline vs. ZD, p = 0.295). (F) The average freezing during ITI1, ITI2, and ITI3. The white and blue bars indicate the average freezing of saline-infused mice (n = 9) and ZD7288 (ZD)-infused mice (n = 11), respectively (independent samples Student’s t test, p = 0.127). (G) Average percentage of freezing during the context test (saline, n = 9 mice; ZD, n = 11 mice; independent samples Student’s t test, p = 0.032). (H) Average percentage of freezing during the cue test (saline, n = 9 mice; ZD, n = 11 mice; independent samples Student’s t test, p = 0.025). (I) Average percentage of freezing during the 2 week context test (saline, n = 9 mice; ZD, n = 11 mice; independent samples Student’s t test, p = 0.059). (J) Average percentage of freezing during the 2 week cue test (saline, n = 9 mice; ZD, n = 11 mice; independent samples Student’s t test, p = 0.023). Data are presented as mean ± SEM. Violin plots range from the 1st through the 99th percentile. Asterisks denote the means, whereas the horizontal bars denote the medians. (K) Percentage of freezing during BL, ITI1, ITI2, and ITI3 of saline- and XE991-injected (1 mg/kg) mice on the training day. The black and blue lines indicate the average percentage of freezing of saline-infused mice (n = 8) and XE991-injected mice (n = 7), respectively (two-way mixed ANOVA followed by post hoc tests with Holm corrections; BL, saline vs. XE991, p = 1.000; ITI1, saline vs. XE991, p = 1.000; ITI2, saline vs. XE991, p = 1.000; ITI3, saline vs. XE991, p = 1.000). (L) The average freezing during ITI1, ITI2, and ITI3. The white and blue bars indicate the average freezing of saline-infused mice (n = 8) and XE991 (XE)-infused mice (n = 7), respectively (independent samples Student’s t test, p = 0.462). (M) Average percentage of freezing during the context test (saline, n = 8 mice; XE, n = 8 mice; independent samples Student’s t test, p = 0.032). (N) Average percentage freezing during the cue test (saline, n = 8 mice; XE, n = 8 mice; independent samples Student’s t test, p = 0.025).

Article Snippet: On the training day, mice were placed in the fear conditioning apparatus (Med Associates, NIR-018SD or Lafayette Instrument 80014CGAT) and were presented with three blocks of tone, trace, and shock after a baseline period of 160 s. For trace conditioning, the tone (20 s) and shock (2 s) presentations were separated by 20 s. For delay conditioning, shock (2 s) was presented during the last 2 s of the 20 s-tone delivery.

Techniques: Saline, Injection